Ttrem1(TTR)Kymm
Endonuclease-mediated Allele Detail
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Symbol: |
Ttrem1(TTR)Kymm |
Name: |
transthyretin; endonuclease-mediated mutation 1, Ken-ichi Yamamura |
MGI ID: |
MGI:6885604 |
Synonyms: |
TtrhTTRV30exon, TtrhV30 |
Gene: |
Ttr Location: Chr18:20798337-20807378 bp, + strand Genetic Position: Chr18, 11.47 cM
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Alliance: |
Ttrem1(TTR)Kymm page
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Germline Transmission: |
Earliest citation of germline transmission:
J:320726
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Parent Cell Line: |
RENKA (ES Cell)
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Strain of Origin: |
C57BL/6N
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Allele Type: |
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Endonuclease-mediated (Humanized sequence, Inserted expressed sequence) |
Mutation: |
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Insertion
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Ttrem1(TTR)Kymm expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
TTR (7276) |
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Human exons, mouse introns |
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Mutation details: CRISPR/Cas9 technology using two gRNAs, one for exon 1 and one for exon 4, and a single donor vector carrying the whole gene in which mouse exons were replaced with human exons but with mouse introns, replaced the endogenous gene with this exon-humanized TTR gene. The a6221 clone showed germline transmission and no gross rearrangement or deletion and sequencing confirmed that all exons have human sequencing.
(J:320726)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Ttr Mutation: |
44 strains or lines available
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Original: |
J:320726 Li Z, et al., TTR exon-humanized mouse optimal for verifying new therapies for FAP. Biochem Biophys Res Commun. 2022 Feb 10;599:69-74 |
All: |
1 reference(s) |
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