Ostn^tm1Ncvc
Targeted Allele Detail

Summary

• Symbol: Ostn^tm1Ncvc
• Name: osteocrin; targeted mutation 1, National Cerebral and Cardiovascular Center
• MGI ID: MGI:6877071
• Synonyms: Ostn^LacZ, OSTN^{tm1a(KOMP)ncvc}
• Gene: Ostn Location: Chr16:27126239-27169962 bp, + strand Genetic Position: Chr16, 18.88 cM
• Alliance: Ostn^tm1Ncvc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:320628
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Targeting vector PG00061_Z_2_D09 from KOMP project was electroporated into ES cells. The targeting cassette is composed of En2 intron/splice-acceptor/exon, an IRES, the beta-galactosidase (LacZ) gene, a polyadenylation signal (pA) a loxP site, a beta-actin promoter, the neomycin-resistance gene (neo), a second pA site, second FRT site, and second loxP site. A third loxP site was inserted downstream of exon 3. In situ hybridization confirmed absence of mRNA in homozygotes. (J:320628)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ostn Mutation: 14 strains or lines available

References

• Original: J:320628 Watanabe-Takano H, et al., Mechanical load regulates bone growth via periosteal Osteocrin. Cell Rep. 2021 Jul 13;36(2):109380
• All: 3 reference(s)