Gt(ROSA)26Sorem25(KRT18-ACE2)Mvw
Endonuclease-mediated Allele Detail
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Symbol: |
Gt(ROSA)26Sorem25(KRT18-ACE2)Mvw |
Name: |
gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 25, Michael V Wiles |
MGI ID: |
MGI:6877060 |
Synonyms: |
RMCE[K18-HuACE2] |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sorem25(KRT18-ACE2)Mvw page
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Allele Type: |
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Endonuclease-mediated (Epitope tag, Humanized sequence, Inserted expressed sequence) |
Mutation: |
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Insertion
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Gt(ROSA)26Sorem25(KRT18-ACE2)Mvw expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
ACE2 (59272) |
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Mutation details: CRISPR/cas9 genome editing and RMCE (recombinase-mediated cassette exchange) are utilized to insert a human keratin 18 promoter directing expression of human ACE2 followed a C-terminal FLAG epitope tag into a modified Gt(ROSA)26Sor (B6.RosaBxb-GT/GA) locus. The C57BL/6J-derived zygotes carrying the Gt(ROSA)26Sor (RosaBxb-GT/GA) allele were previously targeted to insert a dual Bxb1 attP-site "safe harbor landing pad" in the first intron of Gt(ROSA)26Sor. The landing pad contains a 48 bp Bxb1 attP-GT sequence and a 48 bp Bxb1 attP-GA sequence separated by a 240 bp spacer of native Gt(ROSA)26Sor sequence. The DNA donor plasmid, carrying two heterologous attB sites and donor transgene, was then microinjected with Bxb1 integrase into the RMCE-ready zygotes (attP-GT/GA).
(J:322700)
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Original: |
J:322700 Low BE, et al., Efficient targeted transgenesis of large donor DNA into multiple mouse genetic backgrounds using bacteriophage Bxb1 integrase. Sci Rep. 2022 Mar 31;12(1):5424 |
All: |
1 reference(s) |
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