Prkce^tm2.1Msg
Targeted Allele Detail

Summary

• Symbol: Prkce^tm2.1Msg
• Name: protein kinase C, epsilon; targeted mutation 2.1, Robert Messing
• MGI ID: MGI:6867839
• Synonyms: AS-PKCepsilon
• Gene: Prkce Location: Chr17:86475213-86965347 bp, + strand Genetic Position: Chr17, 56.74 cM
• Alliance: Prkce^tm2.1Msg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:318798
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: The targeting construct introduced the mutation that results in the amino acid substitution of methionine with alanine at position 486 (M486A). This mutation produces an ATP analog-sensitive (AS) protein. Cre-mediated recombination removed the floxed neomycin resistance cassette inserted downstream of the modified exon 11. (J:318798)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkce Mutation: 40 strains or lines available

References

• Original: J:318798 Maiya R, et al., Selective chemical genetic inhibition of protein kinase C epsilon reduces ethanol consumption in mice. Neuropharmacology. 2016 Aug;107:40-48
• All: 1 reference(s)