Creld2^tm1.1Emass
Targeted Allele Detail

Summary

• Symbol: Creld2^tm1.1Emass
• Name: cysteine-rich with EGF-like domains 2; targeted mutation 1.1, Elvira Mass
• MGI ID: MGI:6836725
• Synonyms: Creld2^eGFP
• Gene: Creld2 Location: Chr15:88703849-88710884 bp, + strand Genetic Position: Chr15, 44.31 cM
• Alliance: Creld2^tm1.1Emass page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:316411
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intragenic deletion
• Mutation details: The targeting vector is designed to replace the entire coding region of the gene with an enhanced green fluorescent protein (EGFP) sequence. An FRT-flanked neomycin resistance (neo) cassette with a polyadenylation signal (polyA) in reverse orientation to the gene were inserted into the 3' UTR. FLP-mediated recombination removed the FRT-flanked neo cassette. Immunoblot analysis confirmed the absence of protein expression in various tissues of homozygous mutant mice. (J:316411)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Creld2 Mutation: 35 strains or lines available

References

• Original: J:316411 Kern P, et al., Creld2 function during unfolded protein response is essential for liver metabolism homeostasis. FASEB J. 2021 Oct;35(10):e21939
• All: 1 reference(s)