Col1a1tm1(tetO-cas9*)Jnxu
Targeted Allele Detail
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Symbol: |
Col1a1tm1(tetO-cas9*)Jnxu |
Name: |
collagen, type I, alpha 1; targeted mutation 1, Jian Xu |
MGI ID: |
MGI:6835795 |
Synonyms: |
dCas9-KRAB |
Gene: |
Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
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Alliance: |
Col1a1tm1(tetO-cas9*)Jnxu page
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Allele Type: |
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Targeted (Endonuclease, Epitope tag, Inducible) |
Inducer: |
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doxycycline/tetracycline |
Mutation: |
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Insertion
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Mutation details: The plasmid contains a splice acceptor-polyA sequence and the tetracycline responsive element (tetO) upstream of the dCas9-KRAB fusion gene replacing the FRT-flanked PGK-Neo-pA in the 3' UTR of the Col1a1 locus. The dCas9/KRAB fusion gene sequence (dK) is composed of a human codon-optimized, nuclease-deficient Streptococcus pyogenes Cas9 protein synthetic DNA, an HA tag, and two copies of a nuclear localization sequence (NLS), and the repressive chromatin modifier domain mRNA from the Kruppel associated box (KRAB) domain of the human zinc-finger protein 10 gene (ZNF10)
(J:283937)
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Original: |
J:283937 Li K, et al., Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing. Nat Commun. 2020 Jan 24;11(1):485 |
All: |
2 reference(s) |
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