Col1a1^{tm1(tetO-cas9*)Jnxu}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-cas9*)Jnxu}
• Name: collagen, type I, alpha 1; targeted mutation 1, Jian Xu
• MGI ID: MGI:6835795
• Synonyms: dCas9-KRAB
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-cas9*)Jnxu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:283937
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Endonuclease, Epitope tag, Inducible)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The plasmid contains a splice acceptor-polyA sequence and the tetracycline responsive element (tetO) upstream of the dCas9-KRAB fusion gene replacing the FRT-flanked PGK-Neo-pA in the 3' UTR of the Col1a1 locus. The dCas9/KRAB fusion gene sequence (dK) is composed of a human codon-optimized, nuclease-deficient Streptococcus pyogenes Cas9 protein synthetic DNA, an HA tag, and two copies of a nuclear localization sequence (NLS), and the repressive chromatin modifier domain mRNA from the Kruppel associated box (KRAB) domain of the human zinc-finger protein 10 gene (ZNF10) (J:283937)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

References

• Original: J:283937 Li K, et al., Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing. Nat Commun. 2020 Jan 24;11(1):485
• All: 2 reference(s)