Col1a1^{tm4(tetO-Kras*G12V)Jvd}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm4(tetO-Kras*G12V)Jvd}
• Name: collagen, type I, alpha 1; targeted mutation 4, Jan MA van Deursen
• MGI ID: MGI:6835670
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm4(tetO-Kras*G12V)Jvd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:313419
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: RMCE on KH2 cells replaced the FRT-flanked neo cassette with a cassette that contains a splice acceptor, polyadenylation sequence and the tetracycline promoter driving inducible expression of an HA-tagged cDNA for mouse Kras with the amino acid substitution of glycine with valine at position 12 followed by a polyadenylation sequence and hygromycin resistance cassette with an FRT site. (J:313419)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

References

• Original: J:313419 Sturmlechner I, et al., p21 produces a bioactive secretome that places stressed cells under immunosurveillance. Science. 2021 Oct 29;374(6567):eabb3420
• All: 1 reference(s)