Snca^em1Mwcr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Snca^em1Mwcr
• Name: synuclein, alpha; endonuclease-mediated mutation 1, Maxime Rousseaux
• MGI ID: MGI:6834000
• Synonyms: Snca^NLS
• Gene: Snca Location: Chr6:60708559-60806839 bp, - strand Genetic Position: Chr6, 29.15 cM
• Alliance: Snca^em1Mwcr page

Mutation origin

• Strain of Origin: FVB/N

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 genome editing is used to insert a nuclear localization signal (NLS) and FLAG tag. The single-stranded oligonucleotide donor DNA template contains the following sequence: CACTGTGAAGCAGACAGTTGATATCTGTCACTTCACTGACAAGGCATGCTGTTATTATTTTCTTTTTCTGATATTAGGAAGGCTACCAAGACTATGAGCCTGAAGCCGACTACAAGGACGACGACGACAAGTAAGAATGTCATTGCACCCAATCTCCTAAGATCTGCCGGCTGCTCTTCCATGGCGTACAAGTGCTCAGT. (J:351405)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Snca Mutation: 44 strains or lines available

References

• Original: J:351405 Geertsma HM, et al., Constitutive nuclear accumulation of endogenous alpha-synuclein in mice causes motor impairment and cortical dysfunction, independent of protein aggregation. Hum Mol Genet. 2022 Oct 28;31(21):3613-3628
• All: 3 reference(s)