Igs7^{tm1(tetO-mCardinal,-L_RABVS)Wick}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm1(tetO-mCardinal,-L_RABVS)Wick}
• Name: intergenic site 7; targeted mutation 1, Ian Wickersham
• MGI ID: MGI:6832819
• Synonyms: TRE-CB, TRE-tight-mCardinal-P2A-B19L
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm1(tetO-mCardinal,-L_RABVS)Wick} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:367020
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The targeting construct contains a tetracycline-responsive element (TRE; tetO) directing expression of an mCardinal (a basic far red fluorescent protein) and glycoprotein-deleted SAD-B19 rabies virus large structural protein RNA-directed RNA polymerase GTP phosphohydrolase (L_RABVS) variant B19L, separated by a picornavirus 2A element (P2A). The plasmid was then used for FLP-mediated targeted insertion into the Igs7 locus in Ai99 Flp-in embryonic stem (ES) cells derived from (129S6/SvEvTac x C57BL/6)F1 background G4 ES cells. (J:367020)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 76 strains or lines available

References

• Original: J:367020 Jin L, et al., Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses. Nat Neurosci. 2024 Feb;27(2):373-383
• All: 1 reference(s)