Ewsr1^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ewsr1^{tm1d(EUCOMM)Wtsi}
• Name: Ewing sarcoma breakpoint region 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6830504
• Gene: Ewsr1 Location: Chr11:5019689-5049266 bp, - strand Genetic Position: Chr11, 3.22 cM, cytoband A1-A3
• Alliance: Ewsr1^{tm1d(EUCOMM)Wtsi} page
• IMPC: Ewsr1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:314468
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Ewsr1 The L1L2_Bact_P cassette was inserted at position 5041734 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 5042321. The critical exon(s) were thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. (J:314468)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ewsr1 Mutation: 92 strains or lines available

References

• Original: J:314468 Tian H, et al., Mouse EWSR1 is crucial for spermatid post-meiotic transcription and spermiogenesis. Development. 2021 Jun 1;148(11):dev199414
• All: 1 reference(s)