Gt(ROSA)26Sor^{tm5.2(CAG-ZsGreen)Jcbr}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm5.2(CAG-ZsGreen)Jcbr}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 5.2, Jens C Bruning
• MGI ID: MGI:6828612
• Synonyms: R26-rSr-ZsGreen
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm5.2(CAG-ZsGreen)Jcbr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:314806
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A transgene containing from 5' to 3', a loxP-flanked neo stop cassette, a rox-flanked stop cassette, and a 2A-NLS-ZsGreen and driven by the CAGGS promoter (chicken beta actin promoter with CMV enhancers) was inserted into intron 1. The mice with Gt(ROSA)26Sor^{tm1(CAG-ZsGreen)Hamm} were crossed with germline cre deleter mice to remove the loxP-flanked neo stop cassette. An intervening rox-flanked stop cassette prevents expression of the ZsGreen except in the presence of dre when the rox-flanked stop cassette is excised. (J:314806)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:314806 Biglari N, et al., Functionally distinct POMC-expressing neuron subpopulations in hypothalamus revealed by intersectional targeting. Nat Neurosci. 2021 Jul;24(7):913-929
• All: 2 reference(s)