Pln^{tm1.1(PLN)Fstil}
Targeted Allele Detail

Summary

• Symbol: Pln^{tm1.1(PLN)Fstil}
• Name: phospholamban; targeted mutation 1.1, Francesca Stillitano
• MGI ID: MGI:6827284
• Synonyms: hPLN^WT
• Gene: Pln Location: Chr10:53213782-53222095 bp, + strand Genetic Position: Chr10, 27.11 cM, cytoband B3
• Alliance: Pln^{tm1.1(PLN)Fstil} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:314571
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Pln^{tm1.1(PLN)Fstil} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PLN (5350)

  Pln	(MGI:97622)

• Mutation details: The targeting vector contains from 5' to 3'; a loxP site, an H2B-GFP sequence with 4x polyA sequence, an FRT-flanked neomycin selection cassette, a second loxP site, and followed by a human PLN gene inserted into the start codon of exon 2. The insertion results in a knock-out of the mouse Pln gene. Cre-mediated recombination removes the floxed GFP and neo cassette. (J:314571)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pln Mutation: 16 strains or lines available

References

• Original: J:314571 Raad N, et al., Arrhythmia Mechanism and Dynamics in a Humanized Mouse Model of Inherited Cardiomyopathy Caused by Phospholamban R14del Mutation. Circulation. 2021 Aug 10;144(6):441-454
• All: 3 reference(s)