Tie1^em1Lchw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tie1^em1Lchw
• Name: tyrosine kinase with immunoglobulin-like and EGF-like domains 1; endonuclease-mediated mutation 1, Linda C Hsieh-Wilson
• MGI ID: MGI:6814668
• Synonyms: Tie1-2A, Tie1^2A
• Gene: Tie1 Location: Chr4:118328388-118347046 bp, - strand Genetic Position: Chr4, 54.67 cM
• Alliance: Tie1^em1Lchw page

Mutation origin

• Strain of Origin: (C57BL/6J x SJL/J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 technology changed arginine codons 38 (CGT) and 80 (CGC) to alanine (GCC) (p.R38A, p.R82A). Silent mutations to remove the NGG protospacer adjacent motif and introduce restriction enzyme sites were also introduced. These mutations result in loss of heparan sulfate-Tie1 binding. (J:314106)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tie1 Mutation: 69 strains or lines available

References

• Original: J:314106 Griffin ME, et al., Sulfated glycans engage the Ang-Tie pathway to regulate vascular development. Nat Chem Biol. 2021 Feb;17(2):178-186
• All: 1 reference(s)