Ptpn6^tm1.1Mal
Targeted Allele Detail

Summary

• Symbol: Ptpn6^tm1.1Mal
• Name: protein tyrosine phosphatase, non-receptor type 6; targeted mutation 1.1, Bernard Malissen
• MGI ID: MGI:6810179
• Synonyms: Ptpn6^tm1Mal, SHP1^OST
• Gene: Ptpn6 Location: Chr6:124697670-124715672 bp, - strand Genetic Position: Chr6, 59.17 cM
• Alliance: Ptpn6^tm1.1Mal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282407
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Epitope tag)
• Mutation: Insertion
• Mutation details: Sequence for a Gly-Ser-Gly spacer, a OneSTrEPtag (OST), two STOP codons, a loxP site, the mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) promoter, the cre recombinase gene, a neomycin resistance gene cassette, and a second loxP site, was inserted in-frame at the 3 end of the gene coding sequence. Cre-mediated self-excision removed the cre+neo cassette in the male germline. The fusion of the OST to the protein can be used in affinity purification. (J:282407)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptpn6 Mutation: 47 strains or lines available

References

• Original: J:282407 Voisinne G, et al., Quantitative interactomics in primary T cells unveils TCR signal diversification extent and dynamics. Nat Immunol. 2019 Nov;20(11):1530-1541
• All: 1 reference(s)