En1tm1.1(flpo)Jbb
Targeted Allele Detail
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Symbol: |
En1tm1.1(flpo)Jbb |
Name: |
engrailed 1; targeted mutation 1.1, Jay B Bikoff |
MGI ID: |
MGI:6810135 |
Synonyms: |
En1-Flpo, En1::Flpo |
Gene: |
En1 Location: Chr1:120530246-120535719 bp, + strand Genetic Position: Chr1, 52.74 cM, cytoband C2-qter
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Alliance: |
En1tm1.1(flpo)Jbb page
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Germline Transmission: |
Earliest citation of germline transmission:
J:313825
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
Not Specified
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Allele Type: |
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Targeted (Null/knockout, Recombinase) |
Mutation: |
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Insertion
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En1tm1.1(flpo)Jbb expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
En1 (MGI:95389) |
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Mutation details: Flpo, a codon-optimized version of Flp recombinase was inserted into the ATG in the 1st exon of the En1 genomic locus. Positive ES cell clones were screened by Southern blot analysis and microinjected into blastocysts, and the resulting chimeric mice were crossed to C57BL/6J females. The neomycin selectable cassette was removed using Protamine::Cre mice.
(J:313825)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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En1
(mouse)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any En1 Mutation: |
32 strains or lines available
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Original: |
J:313825 Lane AR, et al., Genetic targeting of adult Renshaw cells using a Calbindin 1 destabilized Cre allele for intersection with Parvalbumin or Engrailed1. Sci Rep. 2021 Oct 6;11(1):19861 |
All: |
1 reference(s) |
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