Gt(ROSA)26Sor^{em1(CAG-Cd63/EmGFP)Adly}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-Cd63/EmGFP)Adly}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Andrew Dudley
• MGI ID: MGI:6798105
• Synonyms: CD63-emGFP^{loxP/stop/loxP}, CD63-emGFP^l/s/l
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-Cd63/EmGFP)Adly} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em1(CAG-Cd63/EmGFP)Adly} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Cd63 (MGI:99529)	fused to an EmGFP

• Mutation details: Plasmids encoding a guide RNA (GGAGTTGCAGATCACGA) are designed to insert an FRT site, a neo cassette, and a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), followed by a second FRT site, into intron 1 of the Gt(ROSA)26Sor locus. A floxed STOP cassette followed by a mouse CD63 antigen (Cd63) sequence fused to an emerald green fluorescent protein (emGFP) was inserted downstream of the FRT flanked CAG. (J:292635)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

References

• Original: J:292635 McCann JV, et al., Reporter mice for isolating and auditing cell type-specific extracellular vesicles in vivo. Genesis. 2020 Jul;58(7):e23369
• All: 1 reference(s)