Syne3^tm1.2Nki
Targeted Allele Detail

Summary

• Symbol: Syne3^tm1.2Nki
• Name: spectrin repeat containing, nuclear envelope family member 3; targeted mutation 1.2, The Netherlands Cancer Institute
• MGI ID: MGI:6798091
• Gene: Syne3 Location: Chr12:104896192-104976068 bp, - strand Genetic Position: Chr12, 54.97 cM
• Alliance: Syne3^tm1.2Nki page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:312557
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting construct introduced a loxP site upstream of exon 2 and an FRT-flanked puromycin resistance cassette with a 3 loxP site downstream of exon 2. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exon 2 floxed. Western blot analysis confirmed that both the alpha and beta isoform are no longer expressed. (J:312557)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Syne3 Mutation: 42 strains or lines available

References

• Original: J:312557 Ketema M, et al., Nesprin-3 connects plectin and vimentin to the nuclear envelope of Sertoli cells but is not required for Sertoli cell function in spermatogenesis. Mol Biol Cell. 2013 Aug;24(15):2454-66
• All: 1 reference(s)