Tbx5^{tm1.1(cre/ERT2)Bbr}
Targeted Allele Detail

Summary

• Symbol: Tbx5^{tm1.1(cre/ERT2)Bbr}
• Name: T-box 5; targeted mutation 1.1, Benoit G Bruneau
• MGI ID: MGI:6790619
• Synonyms: Tbx5^CreERT2
• Gene: Tbx5 Location: Chr5:119970733-120023284 bp, + strand Genetic Position: Chr5, 60.42 cM
• Alliance: Tbx5^{tm1.1(cre/ERT2)Bbr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232545
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Epitope tag, Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tbx5^{tm1.1(cre/ERT2)Bbr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tbx5 (MGI:102541)

• Mutation details: A cDNA for Cre/ERT2, followed by an IRES element, a Kozak consensus sequence, and a 2X FLAG epitope sequence was inserted in-frame into exon 2 of the gene, upstream of the endogenous translation start site. At 40bp downstream of exon 2, cloning sites (NotI and AflII) were added and a PGK-EM7-Neo-polyA cassette, flanked by Frt sites. The neo cassette was removed by crossing with Flpe expressing mice. (J:232545)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tbx5 (mouse) Summary of all recombinase alleles driven by Tbx5. Comparative matrix view of recombinase activities Alliance mouse Tbx5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbx5 Mutation: 28 strains or lines available

References

• Original: J:232545 Devine WP, et al., Early patterning and specification of cardiac progenitors in gastrulating mesoderm. Elife. 2014;3
• All: 2 reference(s)