Tg(Cdkn2a/luc/RFP/TK)1Cmps
Transgene Detail

Summary

• Symbol: Tg(Cdkn2a/luc/RFP/TK)1Cmps
• Name: transgene insertion 1, Judith Campisi
• MGI ID: MGI:6766435
• Synonyms: p16-3MR
• Transgene: Tg(Cdkn2a/luc/RFP/TK)1Cmps Location: unknown Genetic Position: Chr13, Syntenic
• Alliance: Tg(Cdkn2a/luc/RFP/TK)1Cmps page

Transgene origin

• Strain of Origin: C57BL/6 x 129

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: A construct consisting of a bioluminescence synthetic Renilla luciferase (hrl) reporter gene, a reporter gene encoding the monomeric red fluorescence protein (mrfp1), and a mutant herpes simplex virus type 1 sr39 thymidine kinase [HSV1-truncated sr39tk (ttk) and a PET reporter gene ("3MR") was inserted in-frame with Cdkn2a exon 2 in the mouse BAC clone RP24-322D20. Expression of this construct results a fusion protein containing the first 62 amino acids of the encoded protein and the 3MR reporters, but no full-length WT protein. Insertion of the 3MR cDNA also resulted in a stop codon in exon 2 to prevent expression from the BAC gene. This transgene is designed to identify, isolate, and selectively kill senescent cells. (J:296170)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:296170 Demaria M, et al., An essential role for senescent cells in optimal wound healing through secretion of PDGF-AA. Dev Cell. 2014 Dec 22;31(6):722-33
• All: 27 reference(s)