Ucp1^em1Hohu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ucp1^em1Hohu
• Name: uncoupling protein 1 (mitochondrial, proton carrier); endonuclease-mediated mutation 1, Hideyo Ohuchi
• MGI ID: MGI:6763933
• Synonyms: Ucp1^{em1(EGFP)Hohu}
• Gene: Ucp1 Location: Chr8:84016981-84025081 bp, + strand Genetic Position: Chr8, 39.65 cM
• Alliance: Ucp1^em1Hohu page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: CRISPR-targeting inserted the EGFP reporter gene and a 2A peptide as in-frame gene fusion long-single strand ODN (lsODN) structure in exon 1 near the start codon. One pup carrying EGFP in the Ucp1 locus (#11) was obtained and established. DNA sequencing of the offspring showed that insertion resulted in a frameshift mutation of the Ucp1 gene. Western blot of homozygous mice confirmed the absence of Ucp1 protein. (J:310472)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ucp1 Mutation: 46 strains or lines available

References

• Original: J:310472 Fujita H, et al., UCP1 expression in the mouse adrenal gland is not upregulated by thermogenic conditions. Biochem Biophys Res Commun. 2021 Aug 20;566:184-189
• All: 1 reference(s)