Ddx25tm2.1(DDX25*R242H)Mld
Targeted Allele Detail
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Symbol: |
Ddx25tm2.1(DDX25*R242H)Mld |
Name: |
DEAD box helicase 25; targeted mutation 2.1, Maria L Dufau |
MGI ID: |
MGI:6759011 |
Synonyms: |
GRTH-KIh |
Gene: |
Ddx25 Location: Chr9:35453144-35469766 bp, - strand Genetic Position: Chr9, 20.31 cM, cytoband A3-A5
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Alliance: |
Ddx25tm2.1(DDX25*R242H)Mld page
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Germline Transmission: |
Earliest citation of germline transmission:
J:291302
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
129/SvJ
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Allele Type: |
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Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
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Ddx25tm2.1(DDX25*R242H)Mld expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
DDX25 (29118) |
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human cDNA containing R242H mutation |
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Mutation details: A cassette consisting of mouse exon 1, EGFP reporter sequence, loxP site, 2A self-cleaving peptide sequence, human DDX25 cDNA containing an arginine to histidine substitution at amino acid 242, SV40 sequence, loxP site, FRT-flanked PGK-neomycin selection cassette, and third loxP site replaced exons 1-5 of the gene. Flp-mediated recombination removed the neomycin cassette. The R242H variant is found in Japanese infertile men.
(J:291302)
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Original: |
J:291302 Kavarthapu R, et al., Targeted Knock-In Mice with a Human Mutation in GRTH/DDX25 Reveals the Essential Role of Phosphorylated GRTH in Spermatid Development during Spermatogenesis. Hum Mol Genet. 2019 Apr 22; |
All: |
4 reference(s) |
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