Gt(ROSA)26Sor^em7Mvw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^em7Mvw
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 7, Michael Wiles
• MGI ID: MGI:6757188
• Synonyms: B6.RosaBxb-GT/GA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^em7Mvw page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (RMCE-ready)
• Mutation: Insertion
• Mutation details: Using CRISPR-Cas9 and oligo-mediated homology directed repair, two heterologous attachment sites, Bxb1 attP-GT and Bxb1 attP-GA, were inserted into intron 1-2, providing cognate attachment and recombination sites for Bxb1 attB-GT and Bxb1 attB-GA, for unidirectional, single-copy donor transgenic integration mediated by Bxb1 Integrase. (J:188991, J:322700)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1098 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 4 reference(s)