Cmpk2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cmpk2^{tm1c(KOMP)Wtsi}
• Name: cytidine/uridine monophosphate kinase 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6753388
• Gene: Cmpk2 Location: Chr12:26519203-26529836 bp, + strand Genetic Position: Chr12, 9.69 cM
• Alliance: Cmpk2^{tm1c(KOMP)Wtsi} page
• IMPC: Cmpk2 gene page

Mutation origin

• Mutant Cell Line: EPD0531_3_F03
• Germline Transmission: Earliest citation of germline transmission: J:308159
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 26523571 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 26524378. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cmpk2 Mutation: 30 strains or lines available

References

• Original: J:308159 Xian H, et al., Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation. Immunity. 2021 Jul 13;54(7):1463-1477.e11
• All: 2 reference(s)