Mpi^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mpi^{tm1c(EUCOMM)Wtsi}
• Name: mannose phosphate isomerase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6741023
• Gene: Mpi Location: Chr9:57451539-57460046 bp, - strand Genetic Position: Chr9, 31.05 cM, cytoband C
• Alliance: Mpi^{tm1c(EUCOMM)Wtsi} page
• IMPC: Mpi gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90559
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 57455553 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 57456951. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:90559)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mpi Mutation: 33 strains or lines available

References

• Original: J:90559 The Mammalian Genetics Unit at Harwell, Information obtained from the Mammalian Genetics Unit, Medical Research Council (MRC), Harwell, UK. Unpublished. 2004-2013
• All: 1 reference(s)