Pdgfra^{tm1.1(flpo)Leol}
Targeted Allele Detail

Summary

• Symbol: Pdgfra^{tm1.1(flpo)Leol}
• Name: platelet derived growth factor receptor, alpha polypeptide; targeted mutation 1.1, Lorin E Olson
• MGI ID: MGI:6731084
• Synonyms: aR^deltaK.Flp, Pdgfra^deltaK.Flp
• Gene: Pdgfra Location: Chr5:75312953-75358876 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Pdgfra^{tm1.1(flpo)Leol} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:306160
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Constitutively active, Recombinase)
• Mutations: Insertion, Intragenic deletion
• Pdgfra^{tm1.1(flpo)Leol} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pdgfra (MGI:97530)

• Mutation details: The targeting cassette consisting of a SA.lox.Neo/Stop.lox-PDGFRaK.T2A.Flp cassette replaced the exons 2-4 of the endogenous Pdgfra gene. The PDGFRaK is a mouse Pdgfra gene cDNA with a point mutation (D842V) renders it constitutively active. Cre-mediated recombination removed lox.Neo/Stop.lox cassette from Pdgfra^{tm1(flpo)Leol} enabling the expression of the constitutively active Pdgfra gene with a point mutation (D842V) and the flpo recombinase. (J:306160)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pdgfra (mouse) Summary of all recombinase alleles driven by Pdgfra. Comparative matrix view of recombinase activities Alliance mouse Pdgfra gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdgfra Mutation: 85 strains or lines available

References

• Original: J:306160 Sun C, et al., Mosaic Mutant Analysis Identifies PDGFRalpha/PDGFRbeta as Negative Regulators of Adipogenesis. Cell Stem Cell. 2020 May 7;26(5):707-721.e5
• All: 1 reference(s)