Wfdc2^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Wfdc2^{tm1b(EUCOMM)Hmgu}
• Name: WAP four-disulfide core domain 2; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6728537
• Synonyms: Wfdc2^lacZ
• Gene: Wfdc2 Location: Chr2:164404635-164410430 bp, + strand Genetic Position: Chr2, 85.2 cM, cytoband H3
• Alliance: Wfdc2^{tm1b(EUCOMM)Hmgu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282576
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 164405575 of Chromosome 2 upstream of the critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 164408134. The critical exons 3 and 4 are thus flanked by loxP sites. Cre-mediated recombination removed the drug resistance gene and exons 3 and 4, generating a reporter knockout mouse. (J:282576)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wfdc2 Mutation: 17 strains or lines available

References

• Original: J:282576 Nakajima K, et al., Lack of whey acidic protein (WAP) four-disulfide core domain protease inhibitor 2 (WFDC2) causes neonatal death from respiratory failure in mice. Dis Model Mech. 2019 Nov 12;12(11):dmm040139
• All: 1 reference(s)