Gt(ROSA)26Sor^{tm1(CAG-ECFP*/Venus*)Tpzn}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-ECFP*/Venus*)Tpzn}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Tullio Pozzan
• MGI ID: MGI:6728074
• Synonyms: mt-Cam
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-ECFP*/Venus*)Tpzn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:341541
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed with (from 5' to 3') a ubiquitously expressed CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxP-flanked stop cassette (containing a betageo sequence), and the mt-Cam cDNA. mt-Cam encodes a FRET-based Cameleon calcium sensor (4mtD3cpv) variant containing a codon-optimized elongated mitochondria tag, a donor chromophore enhanced cyan fluorescent protein (ECFP), calcium sensor sequences [calmodulin and a calmodulin-binding domain from myosin light-chain kinase (M13)], and the acceptor chromophore circularly permuted Venus (cpV). This targeting vector is inserted into the Gt(ROSA)26Sor locus in opposite orientation to the Gt(ROSA)26Sor promoter. (J:341541)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

References

• Original: J:341541 Redolfi N, et al., A New Transgenic Mouse Line for Imaging Mitochondrial Calcium Signals. Function (Oxf). 2021;2(3):zqab012
• All: 1 reference(s)