Cct6b^em1Jsha
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cct6b^em1Jsha
• Name: chaperonin containing TCP1 subunit 6B; endonuclease-mediated mutation 1, Jiahao Sha
• MGI ID: MGI:6724571
• Gene: Cct6b Location: Chr11:82610076-82655147 bp, - strand Genetic Position: Chr11, 50.18 cM, cytoband B5
• Alliance: Cct6b^em1Jsha page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 5bp deletion (c.373_377delAACTG) in exon 4, with the glutamate at position 125 as the first amino acid changed, shifting the reading frame, replacing it for a glycine and terminating at position Ter22 (p.Glu125GlyfsTer22). Western blotting and qPCR confirmed the absence of protein and reduced levels of mRNA in the testis, respectively. (J:307593)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cct6b Mutation: 22 strains or lines available

References

• Original: J:307593 Yang P, et al., T-complex protein 1 subunit zeta-2 (CCT6B) deficiency induces murine teratospermia. PeerJ. 2021;9:e11545
• All: 1 reference(s)