Elavl2^tm1.2Ysa
Targeted Allele Detail

Summary

• Symbol: Elavl2^tm1.2Ysa
• Name: ELAV like RNA binding protein 1; targeted mutation 1.2, Yumiko Saga
• MGI ID: MGI:6720236
• Synonyms: Elavl2^delta, Elavl2^deltadeltaNeo
• Gene: Elavl2 Location: Chr4:91139000-91289022 bp, - strand Genetic Position: Chr4, 42.36 cM
• Alliance: Elavl2^tm1.2Ysa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282136
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: By homologous recombination targeting in ES cells, the exon (ENSMUSE00001275521) including the start codon was flanked by loxP sequences and was followed by a Pgk-neo cassette flanked by Frt sequences. The Pgk-neo cassette was removed by crossing with Rosa-Flp mice. Mice were crossed to mice expressing cre in germline to delete the exon containing the start codon. Western blot analysis confirmed the absence of protein expression in newborn ovaries from homozygous mutant females. This is a null allele. (J:282136)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Elavl2 Mutation: 51 strains or lines available

References

• Original: J:282136 Kato Y, et al., ELAVL2-directed RNA regulatory network drives the formation of quiescent primordial follicles. EMBO Rep. 2019 Dec 5;20(12):e48251
• All: 1 reference(s)