Hprt1^{tm2(CAG-ROCK2*/ERT)Bicrq}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm2(CAG-ROCK2*/ERT)Bicrq}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 2, Cancer Research UK Beatson Institute
• MGI ID: MGI:6719402
• Synonyms: Hprt^{tm2(CAG-ROCK2*/ER)Bicrq}, LSL-KD2:ER
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm2(CAG-ROCK2*/ERT)Bicrq} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237624
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Inserted expressed sequence, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Mutation details: The targeting construct inserted upstream of exon 2 a CAG promoter, floxed stop cassette, EGFP reporter gene, human ROCK2 (kinase-dead version) fused to an engineered version of the hormone binding domain of the estrogen receptor (ERT), and the wild-type promoter and exon one of the endogenous gene. (J:237624)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1280 strains or lines available

References

• Original: J:237624 Samuel MS, et al., Tissue-selective expression of a conditionally-active ROCK2-estrogen receptor fusion protein. Genesis. 2016 Dec;54(12):636-646
• All: 1 reference(s)