Hprt1^{tm1(CAG-ROCK2/ERT)Bicrq}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(CAG-ROCK2/ERT)Bicrq}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Cancer Research UK Beatson Institute
• MGI ID: MGI:6719401
• Synonyms: Hprt^{tm1(CAG-ROCK2/ER)Bicrq}, ROCK2:ER
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(CAG-ROCK2/ERT)Bicrq} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237624
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Inserted expressed sequence, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Hprt1^{tm1(CAG-ROCK2/ERT)Bicrq} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ROCK2 (9475)

  Rock2

  (MGI:107926)

• Hprt1^{tm1(CAG-ROCK2/ERT)Bicrq} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The targeting construct inserted upstream of exon 2 a CAG promoter, floxed stop cassette, EGFP reporter gene, human ROCK2 fused to an engineered version of the hormone binding domain of the estrogen receptor (ERT), and the wild-type promoter and exon one of the endogenous gene. (J:237624)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1280 strains or lines available

References

• Original: J:237624 Samuel MS, et al., Tissue-selective expression of a conditionally-active ROCK2-estrogen receptor fusion protein. Genesis. 2016 Dec;54(12):636-646
• All: 2 reference(s)