Adam17^em1Bbl
Endonuclease-mediated Allele Detail

Summary

• Symbol: Adam17^em1Bbl
• Name: a disintegrin and metallopeptidase domain 17; endonuclease-mediated mutation1, Carl Blobel
• MGI ID: MGI:6719382
• Synonyms: Adam17deltacyto
• Gene: Adam17 Location: Chr12:21373510-21423633 bp, - strand Genetic Position: Chr12, 8.3 cM
• Alliance: Adam17^em1Bbl page

Mutation origin

• Strain of Origin: B6(Cg)-Tyr^c-2J/J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Hypomorph)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing is used to insert a hemaglutinin (HA) tag and stop codon into exon 18 (the cytoplasmic domain). Western blot analysis confirms that the mutation results in decreased protein levels in brain, heart, lung, liver and spleen. (J:306272)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Adam17 Mutation: 62 strains or lines available

References

• Original: J:306272 Lora J, et al., Targeted truncation of the ADAM17 cytoplasmic domain in mice results in protein destabilization and a hypomorphic phenotype. J Biol Chem. 2021 May 3;:100733
• All: 1 reference(s)