Cxcr4^{tm1.1(cre/ERT2)Stum}
Targeted Allele Detail

Summary

• Symbol: Cxcr4^{tm1.1(cre/ERT2)Stum}
• Name: C-X-C motif chemokine receptor 4; targeted mutation 1.1, Ralf Stumm
• MGI ID: MGI:6719054
• Synonyms: Cxcr4^CreER, Cxcr4^{CreER(T2)-IRES-eGFP}
• Gene: Cxcr4 Location: Chr1:128515936-128520030 bp, - strand Genetic Position: Chr1, 56.43 cM
• Alliance: Cxcr4^{tm1.1(cre/ERT2)Stum} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:288822
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Cxcr4^{tm1.1(cre/ERT2)Stum} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cxcr4 (MGI:109563)

• Mutation details: The targeting vector consisting of fragments of 2.9 kb, 3.8 kb and 3.3 kb in size containing the distal promoter sequence, the sequence upstream of exon 1, and the sequence downstream of exon 1, respectively, were used to insert a CreER(T2)-IRES-EGFP cassette and an FRT-flanked neomycin cassette in the 5 end of the 3.3-kb fragment. The linearized construct was electroporated into C57BL/6J mouse ES cells. The neomycin cassette was removed by breeding with C57BL/6J mice expressing Flp-recombinase. With CreER inserted into exon 1, Cxcr4CreER does not produce functional Cxcr4 and homozygous mice bearing this allele are fully deficient in Cxcr4, as verified by immunohistochemistry. (J:288822)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cxcr4 (mouse) Summary of all recombinase alleles driven by Cxcr4. Comparative matrix view of recombinase activities Alliance mouse Cxcr4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cxcr4 Mutation: 40 strains or lines available

References

• Original: J:288822 Werner Y, et al., Cxcr4 distinguishes HSC-derived monocytes from microglia and reveals monocyte immune responses to experimental stroke. Nat Neurosci. 2020 Mar;23(3):351-362
• All: 8 reference(s)