Mki67^{tm1.1(cre/ERT2)Bsed}
Targeted Allele Detail

Summary

• Symbol: Mki67^{tm1.1(cre/ERT2)Bsed}
• Name: antigen identified by monoclonal antibody Ki 67; targeted mutation 1.1, Benedict Seddon
• MGI ID: MGI:6718980
• Synonyms: Mki67^{mCherry-CreERT2}
• Gene: Mki67 Location: Chr7:135291513-135318090 bp, - strand Genetic Position: Chr7, 81.32 cM, cytoband F3-F5
• Alliance: Mki67^{tm1.1(cre/ERT2)Bsed} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:301684
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Mki67^{tm1.1(cre/ERT2)Bsed} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mki67 (MGI:106035)

• Mutation details: The allele was generated by targeted replacement of the terminal exon 14 of the Mki67 locus with a modified exon 14 sequences with upstream FRT flanked neomycin cassette, and downstream mCherry fusion construct, IRES sequence, and CreERT2 cDNA. Mice were crossed with actin-FLPe mice to excise the neomycin selection cassette. (J:301684)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mki67 (mouse) Summary of all recombinase alleles driven by Mki67. Comparative matrix view of recombinase activities Alliance mouse Mki67 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mki67 Mutation: 147 strains or lines available

References

• Original: J:301684 Verheijen M, et al., Fate Mapping Quantifies the Dynamics of B Cell Development and Activation throughout Life. Cell Rep. 2020 Nov 17;33(7):108376
• All: 1 reference(s)