Hoxa13^{tm1.1(cre/ERT2)Mtor}
Targeted Allele Detail

Summary

• Symbol: Hoxa13^{tm1.1(cre/ERT2)Mtor}
• Name: homeobox A13; targeted mutation 1.1, Miguel Torres
• MGI ID: MGI:6718975
• Synonyms: Hoxa13^CreER
• Gene: Hoxa13 Location: Chr6:52235833-52237865 bp, - strand Genetic Position: Chr6, 25.41 cM
• Alliance: Hoxa13^{tm1.1(cre/ERT2)Mtor} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:301894
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Hoxa13^{tm1.1(cre/ERT2)Mtor} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Hoxa13 (MGI:96173)

• Mutation details: The targeting vector containing Cre-ERT2-FRT-Neo-FRT-LoxP cassette inserted into exon 1 just downstream of the endogenous ATG site of Hoxa13 gene, replacing 1864 base pairs of the coding region spanning exons 1 and 2 was generated from a C57BL/6 BAC (bacterial artificial chromosome) clone (RPCI23:33N14) using homologous recombination. C57BL/6NTac 129S6/SvEvTac embryonic stem (ES) cells (Taconic) were electroporated with the targeting construct to generate chimera. After germline transmission, the Neo resistance cassette was eliminated by breeding with a Flpe deleter line. (J:301894)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hoxa13 (mouse) Summary of all recombinase alleles driven by Hoxa13. Comparative matrix view of recombinase activities Alliance mouse Hoxa13 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxa13 Mutation: 29 strains or lines available

References

• Original: J:301894 Delgado I, et al., Proximo-distal positional information encoded by an Fgf-regulated gradient of homeodomain transcription factors in the vertebrate limb. Sci Adv. 2020 Jun;6(23):eaaz0742
• All: 1 reference(s)