Meis2^tm1.1Mtor
Targeted Allele Detail

Summary

• Symbol: Meis2^tm1.1Mtor
• Name: Meis homeobox 2; targeted mutation 1.1, Miguel Torres
• MGI ID: MGI:6718974
• Synonyms: Meis2^flox
• Gene: Meis2 Location: Chr2:115693545-115896320 bp, - strand Genetic Position: Chr2, 58.28 cM
• Alliance: Meis2^tm1.1Mtor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:301894
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The targeting vector introduced loxP sites flanking mouse Meis2 exon 3 and FRT-PGK-gb2-neo-FRT cassette. The targeting vector was electroporated into ES cells. Chimeras were generated by ES cell aggregation. The neomycin resistance cassette was eliminated by crossing with a Flpe deleted mouse. Deletion of this exon results in a premature stop codon, which eliminates all functional domains of the Meis2 protein. (J:301894)

Expression

In Mice Carrying this Mutation:

27 assay results 2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Meis2 Mutation: 29 strains or lines available

References

• Original: J:301894 Delgado I, et al., Proximo-distal positional information encoded by an Fgf-regulated gradient of homeodomain transcription factors in the vertebrate limb. Sci Adv. 2020 Jun;6(23):eaaz0742
• All: 4 reference(s)