Eomes^{tm4.1(icre)Rob}
Targeted Allele Detail

Summary

• Symbol: Eomes^{tm4.1(icre)Rob}
• Name: eomesodermin; targeted mutation 3.1, Elizabeth J Robertson
• MGI ID: MGI:6718928
• Synonyms: Eomes^iCre
• Gene: Eomes Location: Chr9:118307259-118315176 bp, + strand Genetic Position: Chr9, 70.21 cM, cytoband F3
• Alliance: Eomes^{tm4.1(icre)Rob} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:306370
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Eomes^{tm4.1(icre)Rob} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Eomes (MGI:1201683)

• Mutation details: A targeting vector introduced the coding sequence for improved Cre recombinase (iCre) coding sequence followed by a polyA cassette and a loxP-flanked neomycin-resistance cassette was introduced into the exon 1 start site of the Eomes gene, resulting in deletion of about 500 base pairs of the 5' coding region. Correctly targeted ES cells were transiently transfected with a plasmid encoding cre to remove the neomycin cassette. The resulting cells were used to generate germline chimeras. (J:306370)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Eomes (mouse) Summary of all recombinase alleles driven by Eomes. Comparative matrix view of recombinase activities Alliance mouse Eomes gene page

Expression

In Mice Carrying this Mutation:

9 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eomes Mutation: 41 strains or lines available

References

• Original: J:306370 Harland LTG, et al., The T-box transcription factor Eomesodermin governs haemogenic competence of yolk sac mesodermal progenitors. Nat Cell Biol. 2021 Jan;23(1):61-74
• All: 1 reference(s)