Gria1^em1Ran
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gria1^em1Ran
• Name: glutamate receptor, ionotropic, AMPA1 (alpha 1); endonuclease-mediated mutation 1, Roger A Nicoll
• MGI ID: MGI:6718618
• Synonyms: HA-deltaCTD GluA1
• Gene: Gria1 Location: Chr11:56902342-57221070 bp, + strand Genetic Position: Chr11, 34.51 cM
• Alliance: Gria1^em1Ran page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Not Applicable)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 technology inserted a hemagglutinin tag sequence followed by four stop codons (HA-Stop sequence) 3' of the four initial amino acids (EFCY) after the last transmembrane helix. This results in the truncation of the entire cytoplasmic tail after the fourth amino acid after the last transmembrane helix. Western blot analysis confirmed the absence of protein containing the cytoplasmic tail domain in homozygotes. (J:306675)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gria1 Mutation: 66 strains or lines available

References

• Original: J:306675 Diaz-Alonso J, et al., Long-term potentiation is independent of the C-tail of the GluA1 AMPA receptor subunit. Elife. 2020 Aug 24;9:e58042
• All: 1 reference(s)