Zfp683^{tm1.1(cre,Hbegf)Kgis}
Targeted Allele Detail

Summary

• Symbol: Zfp683^{tm1.1(cre,Hbegf)Kgis}
• Name: zinc finger protein 683; targeted mutation 1.1, Klaas van Gisbergen
• MGI ID: MGI:6718519
• Synonyms: B6-Tg(Zfp683-tdTomato-P 2A-Cre-P2A-DTR, Zfp683^{tm1.1(cre,DTR)Kgis}
• Gene: Zfp683 Location: Chr4:133781149-133786307 bp, + strand Genetic Position: Chr4, 66.5 cM
• Alliance: Zfp683^{tm1.1(cre,Hbegf)Kgis} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:306904
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout, Recombinase, Reporter)
• Mutations: Insertion, Intergenic deletion
• Zfp683^{tm1.1(cre,Hbegf)Kgis} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Hbegf

• Zfp683^{tm1.1(cre,Hbegf)Kgis} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Zfp683 (MGI:3650254)

• Mutation details: Homologous recombination in ES cells targeted to insert the fluorescent protein tdTomato, cre recombinase and the diphtheria toxin receptor (DTR) separated by P2A sequences (tdTomato-P2A-Cre-P2A-DTR) into the endogenous Zfp683 locus whereby the targeting sequence replaced exons 2, 3 and 4, as well as intronic sequences of the Zfp683 gene (knockin knockout). An FRT-flanked neomycin resistance gene was also introduced. Embryonic stem cell-derived offspring were then bred with FLPO mice to remove neo selection cassette. (J:306904)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Zfp683 (mouse) Summary of all recombinase alleles driven by Zfp683. Comparative matrix view of recombinase activities Alliance mouse Zfp683 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp683 Mutation: 20 strains or lines available

References

• Original: J:306904 Behr FM, et al., Tissue-resident memory CD8(+) T cells shape local and systemic secondary T cell responses. Nat Immunol. 2020 Sep;21(9):1070-1081
• All: 4 reference(s)