Doc2b^{em1(folA/cre)Guru}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Doc2b^{em1(folA/cre)Guru}
• Name: double C2, beta; endonuclease-mediated mutation 1, Channabasavaiah Gurumurthy
• MGI ID: MGI:6718299
• Synonyms: Doc2b^ddCre
• Gene: Doc2b Location: Chr11:75659792-75686875 bp, - strand Genetic Position: Chr11, 45.92 cM
• Alliance: Doc2b^{em1(folA/cre)Guru} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: trimethoprim
• Mutation: Insertion
• Doc2b^{em1(folA/cre)Guru} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Doc2b (MGI:1100497)

• Mutation details: CRISPR/cas9 endonuclease mediated genome editing is used to introduce P2A-trimethoprim(TMP)-inducible cre (ddCre) cassette immediately before the stop codon of Doc2b gene. Cre recombinase is fused to the destabilizing DHFR (or folA) domain, which can be transiently stabilized by the administration of TMP. (J:304624)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Doc2b (mouse) Summary of all recombinase alleles driven by Doc2b. Comparative matrix view of recombinase activities Alliance mouse Doc2b gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Doc2b Mutation: 19 strains or lines available

References

• Original: J:304624 Wu H, et al., Distinct subtypes of proprioceptive dorsal root ganglion neurons regulate adaptive proprioception in mice. Nat Commun. 2021 Feb 15;12(1):1026
• All: 1 reference(s)