Sh2b1^em3Ccsu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sh2b1^em3Ccsu
• Name: SH2B adaptor protein 1; endonuclease-mediated mutation 3, Christin Carter-Su
• MGI ID: MGI:6718275
• Synonyms: alphadeltaKO, Sh2b1^{DELalphadelta}
• Gene: Sh2b1 Location: Chr7:126066166-126074596 bp, - strand Genetic Position: Chr7, 69.06 cM
• Alliance: Sh2b1^em3Ccsu page

Mutation origin

• Strain of Origin: (C57BL/6J x SJL)F2

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9 genome editing is used to delete exon 9, intron 9, the sequence in exon 10 that contains the beta/gamma; stop codons, and the region of exon 10 downstream of the alpha/stop codon. Immunoblot analysis confirms the absence of alpha and delta splice variants and the presence of the beta/gamma proteins in brain lysates. (J:306787)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sh2b1 Mutation: 51 strains or lines available

References

• Original: J:306787 Cote JL, et al., Deletion of the Brain-Specific alpha and delta Isoforms of Adapter Protein SH2B1 Protects Mice From Obesity. Diabetes. 2021 Feb;70(2):400-414
• All: 1 reference(s)