Igs28^{tm2(ACTB-tdTomato,-EGFP)Biat}
Targeted Allele Detail

Summary

• Symbol: Igs28^{tm2(ACTB-tdTomato,-EGFP)Biat}
• Name: intergenic site 28; targeted mutation 2, Biomodels Austria
• MGI ID: MGI:6715982
• Synonyms: MADM-4-TG
• Gene: Igs28 Location: unknown Genetic Position: Chr4, Syntenic
• Alliance: Igs28^{tm2(ACTB-tdTomato,-EGFP)Biat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:306183
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The TG MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a red fluorescent protein (tdTomato; aa 1-3), a beta-globin intronic sequence containing an FRT site and loxP-flanked neomycin resistance gene, the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 274-724), and an SV40 T-antigen poly(A) signal. This entire TG MADM construct was inserted into the "Hipp4" locus on chromosome 4 (between Rab2a and Chd7).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs28 Mutation: 2 strains or lines available

References

• Original: J:306183 Contreras X, et al., A genome-wide library of MADM mice for single-cell genetic mosaic analysis. Cell Rep. 2021 Jun;35
• All: 1 reference(s)