Gt(ROSA)26Sor^{em1.1(CAG-ERT2/Hoxb8)Sykes}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1.1(CAG-ERT2/Hoxb8)Sykes}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1.1, David Sykes
• MGI ID: MGI:6712697
• Synonyms: HOXER
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1.1(CAG-ERT2/Hoxb8)Sykes} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Inserted expressed sequence)
• Inducer: tamoxifen
• Mutation: Insertion
• Gt(ROSA)26Sor^{em1.1(CAG-ERT2/Hoxb8)Sykes} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hoxb8 (MGI:96189)

• Mutation details: CRISPR/cas9 genome editing is used to insert a CAG promoter followed by a loxP:STOP:loxP (LSL) followed by the ER-Hoxb8 fusion protein into the Gt(ROSA)26Sor locus. Transient transfection with cre recombinase removes the LSL and leaves a single loxP site. The ER-Hoxb8 fusion protein comprises the hormone binding domain of the human estrogen receptor alpha fused to the murine Hoxb8 sequence. In this system, the ER-Hoxb8 protein is constitutively expressed; the estrogen receptor fusion renders the protein functionally dependent on estrogen. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)