Mir205^tm1.3Mtm
Targeted Allele Detail

Summary

• Symbol: Mir205^tm1.3Mtm
• Name: microRNA 205; targeted mutation 1.3, Michael McManus
• MGI ID: MGI:6712640
• Synonyms: miR-205 flox
• Gene: Mir205 Location: Chr1:193189771-193189838 bp, - strand Genetic Position: Chr1, 97.82 cM
• Alliance: Mir205^tm1.3Mtm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243203
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop and one loxP site immediately downstream of the microRNA stem loop. Flp-mediation removed the reporter/selection cassette and left the microRNA floxed. (J:243203)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir205 Mutation: 3 strains or lines available

References

• Original: J:243203 Farmer DT, et al., miR-205 is a critical regulator of lacrimal gland development. Dev Biol. 2017 Jul 01;427(1):12-20
• All: 1 reference(s)