Gpr182^{tm2c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Gpr182^{tm2c(KOMP)Wtsi}
• Name: G protein-coupled receptor 182; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6712271
• Synonyms: Gpr182^flox
• Gene: Gpr182 Location: Chr10:127585471-127587667 bp, - strand Genetic Position: Chr10, 75.07 cM
• Alliance: Gpr182^{tm2c(KOMP)Wtsi} page
• IMPC: Gpr182 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:305796
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 127585300 of Chromosome 10 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 127587278. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml . (J:305796)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpr182 Mutation: 23 strains or lines available

References

• Original: J:305796 Le Mercier A, et al., GPR182 is an endothelium-specific atypical chemokine receptor that maintains hematopoietic stem cell homeostasis. Proc Natl Acad Sci U S A. 2021 Apr 27;118(17):e2021596118
• All: 1 reference(s)