Vav1^tm1.1Mal
Targeted Allele Detail

Summary

• Symbol: Vav1^tm1.1Mal
• Name: vav guanine nucleotide exchange factor 1; targeted mutation 1.1, Bernard Malissen
• MGI ID: MGI:6690663
• Synonyms: Vav1^OST, VAV1^OST, Vav1^tm1Mal
• Gene: Vav1 Location: Chr17:57586100-57635031 bp, + strand Genetic Position: Chr17, 29.76 cM
• Alliance: Vav1^tm1.1Mal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:284464
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Epitope tag, No functional change)
• Mutation: Insertion
• Mutation details: Sequence for a Gly-Ser-Gly spacer, a OneSTrEPtag (OST), two STOP codons, a loxP site, the mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) promoter, the cre recombinase gene, a neomycin resistance gene cassette, and a second loxP site, was inserted in-frame at the 3 end of the gene coding sequence in exon 27. Cre-mediated self-excision removed the selection cassette in the male germline. The fusion of the OST to the protein can be used in affinity purification. The presence of this tag does not affect protein expression. (J:284464)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vav1 Mutation: 63 strains or lines available

References

• Original: J:284464 Gaud G, et al., The costimulatory molecule CD226 signals through VAV1 to amplify TCR signals and promote IL-17 production by CD4(+) T cells. Sci Signal. 2018 Jul 10;11(538)
• All: 2 reference(s)