Cbl^tm1.1Mal
Targeted Allele Detail

Summary

• Symbol: Cbl^tm1.1Mal
• Name: Cbl proto-oncogene; targeted mutation 1.1, Bernard Malissen
• MGI ID: MGI:6690659
• Synonyms: Cbl^OST, CBL^OST, Cbl^tm1Mal
• Gene: Cbl Location: Chr9:44054273-44145346 bp, - strand Genetic Position: Chr9, 24.72 cM
• Alliance: Cbl^tm1.1Mal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239186
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Epitope tag, No functional change)
• Mutation: Insertion
• Mutation details: Sequence for a OneSTrEPtag (OST), two STOP codons, a loxP site, the mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) promoter, the cre recombinase gene, a neomycin resistance gene cassette, and a second loxP site, was inserted in-frame at the 3 end of the gene coding sequence in exon 16. Cre-mediated self-excision removed the cre+neo cassette in the male germline. The fusion of the OST to the protein can be used in affinity purification. The presence of this tag does not affect protein expression. (J:239186)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cbl Mutation: 56 strains or lines available

References

• Original: J:239186 Voisinne G, et al., Co-recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR-induced ubiquitylation. Mol Syst Biol. 2016 Jul 29;12(7):876
• All: 2 reference(s)