Ttll3^tm1.2Ics
Targeted Allele Detail

Summary

• Symbol: Ttll3^tm1.2Ics
• Name: tubulin tyrosine ligase-like family, member 3; targeted mutation 1.2, Mouse Clinical Institute
• MGI ID: MGI:6514906
• Gene: Ttll3 Location: Chr6:113366221-113391548 bp, + strand Genetic Position: Chr6, 52.76 cM
• Alliance: Ttll3^tm1.2Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:300009
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_gt0 cassette was inserted at position 113375266 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 113376103. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:300009)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ttll3 Mutation: 84 strains or lines available

References

• Original: J:300009 Gadadhar S, et al., Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility. Science. 2021 Jan 8;371(6525)
• All: 1 reference(s)