Ttll3^tm1.1Ics
Targeted Allele Detail

Summary

• Symbol: Ttll3^tm1.1Ics
• Name: tubulin tyrosine ligase-like family, member 3; targeted mutation 1.1, Mouse Clinical Institute
• MGI ID: MGI:6514905
• Synonyms: Ttll3^flox
• Gene: Ttll3 Location: Chr6:113366221-113391548 bp, + strand Genetic Position: Chr6, 52.76 cM
• Alliance: Ttll3^tm1.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:300009
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 113375266 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 113376103. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:300009)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ttll3 Mutation: 84 strains or lines available

References

• Original: J:300009 Gadadhar S, et al., Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility. Science. 2021 Jan 8;371(6525)
• All: 1 reference(s)