Pbk^em1Xxh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pbk^em1Xxh
• Name: PDZ binding kinase; endonuclease-mediated mutation 1, Xianxin Hua
• MGI ID: MGI:6512044
• Synonyms: Pbk^KI
• Gene: Pbk Location: Chr14:66043337-66055271 bp, + strand Genetic Position: Chr14, 34.36 cM
• Alliance: Pbk^em1Xxh page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/cas9 genome editing is used to change AAA AAG to GCT GCC, resulting in two lysine to alanine changes at amino acids 63 and 64 (K63A and K64A) in exon 5. Two mismatched bases with silence mutations (CGTG to TGTC) that generate a novel Mwol restriction site were mutated directly upstream of amino acid 64. It is unknown if there are any off-target sites present. (J:341516)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pbk Mutation: 35 strains or lines available

References

• Original: J:341516 Ma J, et al., Menin-regulated Pbk controls high fat diet-induced compensatory beta cell proliferation. EMBO Mol Med. 2021 May 7;13(5):e13524
• All: 2 reference(s)